RESEARCH ARTICLE


Distinct Reactivity of Transient Receptor Potential Vanilloid Subtype 1 in a Murine Model of Atopic Dermatitis with Serious Scratching



Yan Xia1, Akane Tanaka*, 1, 2, Kumiko Oida1, Akira Matsuda3, Hyosun Jang1, Yosuke Amagai1, Saori Ishizaka1, Hiroshi Matsuda1, 3
1 Cooperative Major in Advanced Health Science, Graduate School of Bio-Applications and System Engineering, Labo-ratories of
2 Comparative Animal Medicine and
3 Comparative Animal Medicine and eterinary Molecular Pathology and Therapeutics, Division of Animal Life Science, Institute of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.

Article Information


Identifiers and Pagination:

Year: 2014
Volume: 7
First Page: 10
Last Page: 16
Publisher Id: TOALLJ-7-10
DOI: 10.2174/1874838401407010010

Article History:

Received Date: 30/4/2014
Revision Received Date: 3/8/2014
Acceptance Date: 1/9/2014
Electronic publication date: 19/11/2014
Collection year: 2014

© 2014 Xia et al;

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Laboratories of Comparative Animal Medicine and Veterinary Molecular Pathology and Therapeutics, Division of Animal Life Science, Institute of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan.


Abstract

Background:

Abnormality in skin sensitivity may be responsible for unbearable itch in patients with atopic dermatitis (AD).

Objectives:

We evaluated reactivity of NC/Tnd mice, a model for human AD, against various experimental stimulations.

Methods:

Several behavioral tests were performed after external stimuli were applied to NC/Tnd mice. Transient receptor potential vanilloid subtype 1 (TRPV1) reactivity of neuronal cells collected from the dorsal root ganglions (DRG) was analyzed with a Ca++ influx test. Finally, we evaluated suppressive effect of capsaicin on atopic itch of NC/Tnd mice.

Results:

Pain responses to heat, acidic stimulation, and capsaicin injection, which are transduced through TRPV1, were decreased in NC/Tnd mice, when compared to two standard strains BALB/c and C57BL/6 mice. The reactivity of the primary neurons isolated from DRG to capsaicin was markedly reduced in NC/Tnd mice. Topical application of histamine evoked scratching in NC/Tnd mice as well as other two strains; however, the scratching intensities induced by nonhistamine pruritogens were significantly lower in NC/Tnd mice comparing to the two strains. In conventional NC/Tnd mice with AD, topical application of capsaicin reduced the scratching behavior.

Conclusion:

TRPV1 is associated with both pain and itch sensation; however, abnormalities in TRPV1 reactivity may involve in severe itch in NC/Tnd mice.

Keywords: Atopic dermatitis, itch, NC/Tnd mice, TRPV1.